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Stanford laboratory has developed a number of innovative and cutting-edge molecular assays. |
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Molecular Microbiology
Bordetella pertussis nucleic acid amplification:
This test, a real-time PCR assay that detects a unique insertion sequence, developed at Stanford, is the most sensitive test for detection of pertussis or parapertussis. It is usually performed three times per week. A special collection kit, available from the laboratory, is recommended.
MecA gene detection by real-time PCR:
All Staphylococcus aureus and clinically important coagulase-negative staphylococci are tested for the presence of the mecA gene by real-time PCR. This assay is performed on the same day culture is detected. Staphylococcus lugdunensis isolates are also identified by real-time PCR.
Clostridium difficile detection by real-time PCR:
Upon completion of internal validation, the laboratory will routinely perform real-time PCR for rapid and sensitive detection of Clostridium difficile in stool specimen.
Strep Group B screening by real-time PCR:
Real-time PCR, a more sensitive assay compared to culture, for Strep Beta Group B is performed on vaginal/anal swabs after enrichment in selective broth.
Mycobacterial culture identification by multiplex, real-time PCR:
All isolates are rapidly identified with multiplexed, real-time PCR. Ribosomal RNA gene sequencing is used for some non-tuberculous mycobacteria..
Bacterial and fungal identification with gene sequencing:
Isolates not identifiable by routine microbiology methods are identified with gene sequencing.
Pulsed field gel electrophoresis bacterial strain typing:
Bacteria are pelleted, DNA is treated with endonuclease enzymes, the strands are separated, and the DNA strands are subjected to varying waves of electrical current in a polyacrylamide gel. The resulting band patterns are used to define the relatedness among strains. Stanford has experience with numerous species, and we are especially knowledgeable about methicillin-resistant Staphylococcus aureus. To submit strains for testing, contact Micro Lab at 650-723-5294 and ask for “Strain Typing Technologist”
