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Stanford laboratory has developed a number of innovative and cutting-edge molecular assays. |
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Molecular Microbiology
Bordetella pertussis nucleic acid amplification: This test, a real-time PCR assay that detects a unique insertion sequence, developed at Stanford, is the most sensitive test for detection of pertussis or parapertussis. It is usually performed three times per week. A special collection kit, available from the laboratory, is recommended.
Pulsed field gel electrophoresis bacterial strain typing: Bacteria are pelleted, DNA is treated with endonuclease enzymes, the strands are separated, and the DNA strands are subjected to varying waves of electrical current in a polyacrylamide gel. The resulting band patterns are used to define the relatedness among strains. Stanford has experience with numerous species, and we are especially knowledgeable about methicillin-resistant Staphylococcus aureus. To submit strains for testing, contact Micro Lab at 650-723-5294 and ask for “Strain Typing Technologist”
MecA gene detection by PCR: Under special circumstances, staphylococci with inconsistent phenotypical results can be tested for the presence of the mecA gene by real-time PCR. Call the laboratory for the turnaround time for this test.
